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The plane crash on June 12 in Ahmedabad, Gujarat has shook the entire country. Air India flight from Ahmedabad to London crashed a few minutes after the AI-171 takeoff, in which 241 passengers died. Former Gujarat Chief Minister Vijay Rupani also died in this terrible accident. The bodies of the victims of the accident have been burnt so badly that it is impossible to identify them through traditional methods. In such a situation, the method of DNA test is left for identification. Do you know how DNA test is done? Which part of the badly burnt bodies in Ahmedabad plane crash will help in this work? At the same time, how many distant relatives are taken help in this investigation? & Nbsp;

what is DNA test?

DNA ie deoxybonucleic acid test is a scientific technique, which checks the genetic structure of a person and identifies it. The DNA of every person in the world is different and it is found in the nucleus of cells. This technique is especially effective when it is extremely difficult to identify bodies. The bodies have been severely burnt in the Ahmedabad plane crash, due to which DNA test is the main source of identity. & Nbsp;

How DNA Test is done? & Nbsp;

DNA testing is a difficult, but systematic process. & nbsp; It is examined under several stages. & nbsp;

"Text-Align: justify;"> sample collection: samples are first taken for DNA test. After the Ahmedabad plane crash accident, the Gujarat government has made arrangements to keep DNA samples at Bij Medical College. Samples of bones, teeth, or tissue will be taken from the bodies of the dead. They will be matched with their families like parents, children, or siblings of siblings or saliva. & Nbsp;
• How samples are taken from bodies? samples of bones like femur, humus or rib are taken to extract DNA from badly burnt bodies. Apart from this, teeth or remaining tissue are also very effective. Actually, bones and teeth are more important, because on high temperature also DNA is preserved in them. & Nbsp;
• samples from relatives: After this, blood, saliva, or cheek cells (bookkel swabs) are taken from family members. These samples are used for matching the DNA of the deceased. & Nbsp;

cleaning and preparation of samples

It is very difficult to remove DNA from burnt bodies in the accident, because high temperatures and chemicals can destroy DNA. In such a situation, the sample is first cleaned, so that outdoor pollutants like debris or microorganisms can be removed. For this, the sample is washed with tap water and 100% ethanol. Steril instruments are used to grind bones and teeth in powder. & Nbsp;

How is DNA extracted from bones?

Organic extraction process is used to remove DNA. Under this, DNA is separated by putting samples in chemical solution. In the Ahmedabad accident, the bodies have been affected by 1.25 lakh liters of fuel fire. In such a situation, the quality of DNA can be affected. However, new technology manages to extract DNA from burnt remains.

How much DNA is necessary for investigation? & nbsp;

The amount and quality of the extracted DNA is examined through real-time PCR (polymerase chain reaction). In such a situation, it is first ensured whether DNA is enough for examination or not.

How DNA Profiling is?

Short tendom repeats (STRs) are examined in DNA profiles. These are parts of DNA, which are different in every person. After this DNA profile is prepared. About 1000 DNA tests are being done in the Ahmedabad accident, including matching the samples of the dead and their families.

How far relatives go to samples?

The DNA profile of the deceased is mixed with family samples. For this, matching with the DNA of parents or children is considered more accurate, because there is more genetic equality between them. If investigation is being done under an accident like Ahmedabad plane crash, then this process of DNA test may take several days.

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disclaimer: Some information given in the news is based on media reports. Before implementing any suggestion, you must consult the concerned expert.

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